Journal: eLife
Article Title: Co-regulation and function of FOXM1 / RHNO1 bidirectional genes in cancer
doi: 10.7554/eLife.55070
Figure Lengend Snippet: ( A ) Schematic of the F/R-BDP dual luciferase reporter construct. The FOXM1 promoter direction drives Renilla luciferase, and the RHNO1 promoter direction drives firefly luciferase. Secreted embryonic alkaline phosphatase (SEAP) was used as a normalization control for transfection efficiency. ( B ) Correlation of FOXM1 and RHNO1 promoter activity in Fallopian tube epithelium (FTE) (FT282) and high-grade serous carcinoma (HGSC) cell lines (OVCAR5, COV318, OVCAR4, Kuramochi, SNU119). ( C, D ) Correlation of FOXM1 or RHNO1 promoter activity vs. endogenous expression of the cognate gene in FTE (FT282) and HGSC (OVCAR5, COV318, Kuramochi, SNU119) cell lines, as measured by RT-qPCR. In ( B–D ), error bars represent standard deviations. ( E ) Schematic of the F/R-BDP dual fluorescence reporter construct. The FOXM1 promoter direction drives red fluorescent protein (RFP) expression, and the RHNO1 promoter direction drives green fluorescent protein (GFP) expression. ( F ) Fluorescence-activated cell sorting analysis of GFP and RFP fluorescence in 293T cells transfected with the F/R-BDP reporter shown in ( E ). Quadrant 2 (RFP + GFP + cell population) correlation statistics are shown.
Article Snippet: Genetic reagent ( Homo sapiens ) , pGL4 Luc Rluc Empty , Addgene , 64034 , Plasmid.
Techniques: Luciferase, Construct, Transfection, Activity Assay, Expressing, Quantitative RT-PCR, Fluorescence, FACS